Table 2.
Effect of different auxins on in vitro rooting of microshoots of Swertia lawii.
| PGRs | Concentration (mg l−1) | Regeneration frequency (%) | Number of shoots (mean ± SE) | Length of shoots (cm) (mean ± SE) |
|---|---|---|---|---|
| PGR free | 00 | 00 | 0.0 | 0.0 |
| IBA | 1.0 | 100 | 14.7 ± 2.8** | 1.7 ± 0.3** |
| 2.0 | 100 | 16.8 ± 1.8** | 1.4 ± 0.1** | |
| 3.0 | 95 | 16.7 ± 2.4** | 0.8 ± 0.2** | |
| 4.0 | 80 | 08.6 ± 1.7* | 0.4 ± 0.1ns | |
| 5.0 | 65 | 10.0 ± 2.3** | 0.3 ± 0.1ns | |
| IAA | 1.0 | 100 | 15.5 ± 1.2** | 1.5 ± 0.1** |
| 2.0 | 100 | 12.8 ± 0.8** | 1.8 ± 0.2** | |
| 3.0 | 100 | 22.1 ± 2.5** | 2.5 ± 0.3** | |
| 4.0 | 100 | 21.2 ± 1.8** | 2.4 ± 0.1** | |
| 5.0 | 95 | 15.4 ± 1.5** | 2.1 ± 0.1** | |
| NAA | 1.0 | 70 | 14.6 ± 2.9** | 1.5 ± 0.2** |
| 2.0 | 75 | 13.2 ± 3.1** | 1.0 ± 0.2** | |
| 3.0 | 70 | 11.3 ± 2.6** | 0.8 ± 0.2** | |
| 4.0 | 60 | 09.9 ± 2.6* | 0.5 ± 0.1ns | |
| 5.0 | 65 | 07.2 ± 2.0ns | 0.4 ± 0.1ns |
Mean ± S.E. of 30 replicates per treatment and experiments were repeated thrice. The values are significantly different at ns – non significant, *P < 0.05 and **P < 0.01 level when compared by Dunnett multiple comparisons test using one way ANOVA.