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. 2017 Jun 9;12(6):e0179204. doi: 10.1371/journal.pone.0179204

Fig 6. α-MG reduced inflammatory cell migration and cytokines.

Fig 6

(A) Peritoneal macrophages and (B) Raw 264.7 (macrophage) cells were treated with CCL2 (10 ng/ml) or ATCM (adipose tissue-conditioned medium) in the presence and absence of CCR2 inhibitor (10 μM/ml) or α-MG (25 μM/ml) for 24h. The number of transmigrated peritoneal macrophages was measured using a migration assay in the presence and absence of α-MG. Original magnification is x200 (scale bar = 100 μm). (C) mRNA expression of pro-inflammatory cytokines (TNF-α, MCP-1, CCR2, and IL-6) and the anti-inflammatory cytokine IL-10 in Raw264.7 cells. mRNA levels were estimated using real-time PCR. *P < 0.05 compared to control. #P < 0.05 compared to CCL2 or ATCM treated.