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. 2017 May 30;11(5):e0005562. doi: 10.1371/journal.pntd.0005562

Fig 3. Comparison of ELISA reactivity and ROC curves obtained from canine sera against recLdVFA2 and antigen of EIE-LVC kit.

Fig 3

(A) ELISA reactivity obtained from dogs sera against EIE-LVC kit. (B) ELISA reactivity obtained from dogs sera against recLdVFA2 bi-epitope. The ELISA was performed with groups of 101 uninfected dogs (NI), 101 infected with Leishmania (I) and 10 infected with Trypanosoma cruzi (TC). (C) ROC curve obtained from EIE-LVC kit. (D) ROC curve obtained from recLdVFA2 bi-epitope. The ROC curves were used to determine cut-off, sensitivity, specificity and AUC. Data variation expressed as standard error. *Cut-off obtained by ROC curve and #Cut-off obtained according to kit manufacturer.