Fig 8. The position of type II Ca²⁺-binding sites is critical for SiiE function.

The type II Ca²⁺-binding sites of 5 consecutive BIg domains (Δ5_typeII) were removed by D/S exchanges in various positions of SiiE as indicated (A). B) SiiE-dependent invasion of polarized epithelial MDCK cells by Salmonella strains expressing WT and mutant alleles of SiiE. Analysis of SiiE-dependent invasion of polarized cells was performed as described for Fig 2.