Table 2. Protein, cAMP, and NAD(P)H contents of different morphological forms of Benjaminiella poitrasii.
| Stage | Sample | Protein# (mg/g of cells wt. weight) | cAMP* (pmol/g of cells wt. weight) | NAD(P)H** (nmol/g of cells wt. weight) |
|---|---|---|---|---|
| Vegetative | Yeast 12 h | 8.5 | 3000 | 4394 |
| Yeast 24 h | 8.7 | 1840 | 7083 | |
| Hyphae 12 h | 4.2 | 1600 | 3539 | |
| Hyphae 24 h | 5.0 | 1140 | 3103 | |
| Yeast 12h 0.1% YPG, 28°C | 8.6 | 3800 | 4859 | |
| Hyphae 12h 0.1% YPG, 28°C | 4.3 | 1640 | 3111 | |
| Asexual | Sporangiospores 5 d | 2.8 | 1340 | 3342 |
| Sporangiospores 10 d | 2.0 | 700 | 2935 | |
| Sporangiospores 15 d | 1.8 | 540 | 1748 | |
| Sexual | Zygospores 7 d | 5.6 | 1120 | 3321 |
| Zygospores 15 d | 2.8 | 900 | 2648 | |
| Zygospores 30 d | 2.3 | 800 | 2524 |
*cAMP content was estimated by using cAMP enzyme immunoassay 96 well kit from Sigma (Product number CA-201).
** NAD(P)H content was estimated by measuring the absorbance of each sample at 340nm and concentration of NAD(P)H was then determined by interpolation from standard calibration curve.
# Protein was estimated by Bradford method using Bovine serum albumin as standard. 0.1% YPG (yeast extract 0.3%, peptone 0.5%, glucose 0.1%)