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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Bioorg Med Chem. 2016 Sep 30;24(22):6094–6101. doi: 10.1016/j.bmc.2016.09.072

Table 1.

The 20 most highly cleaved sites for BLM and the analogues with the RTGTAY plasmid.
BLM 6′-deoxy-BLM Z BLM Z ZBM
Base % Cleavage Sequence Base % Cleavage Sequence Base % Cleavage Sequence Base % Cleavage Sequence
1 188 2.59 CT GT AT 188 2.96 CT GT AT 188 2.48 CT GT AT 89 3.73 GT GT AC
2 179 2.55 CT GT AA 179 2.55 CT GT AA 179 2.43 CT GT AA 80 3.32 GT GT AT
3 325 2.49 TC GT AT 116 2.52 AT GT AT 386 2.41 TT GT AT 69 3.18 TT GT GT
4 143 2.48 TT GT AA 152 2.50 TT GT AT 152 2.38 TT GT AT 497 3.04 GC GT AA
5 152 2.48 TT GT AT 317 2.48 TT GT AT 325 2.34 TC GT AT 78 3.00 TT GT GT
6 386 2.45 TT GT AT 197 2.44 CT GT AC 143 2.32 TT GT AA 87 2.99 TT GT GT
7 261 2.43 TC GT AA 107 2.40 AT GT AA 197 2.30 CT GT AC 62 2.70 GT GT AG
8 107 2.42 AT GT AA 261 2.37 TC GT AA 317 2.30 TT GT AT 60 2.57 TT GT GT
9 116 2.41 AT GT AT 422 2.36 TT GC CT 261 2.30 TC GT AA 125 2.50 AT GT AC
10 317 2.37 TT GT AT 386 2.34 TT GT AT 116 2.25 AT GT AT 197 2.49 CT GT AC
11 253 2.28 TT GT AA 143 2.33 TT GT AA 253 2.14 TT GT AA 116 2.48 AT GT AT
12 49 2.22 GG GT AA 325 2.30 TC GT AT 107 2.14 AT GT AA 71 2.38 GT GT AA
13 170 2.15 CT GT AG 170 2.23 CT GT AG 134 2.04 TT GT AG 98 2.29 AT GT AG
14 134 2.11 TT GT AG 253 2.17 TT GT AA 422 2.02 TT GC CT 293 2.21 TC GT AC
15 197 2.07 CT GT AC 221 2.15 TT GT AG 161 2.00 TT GT AC 161 2.19 TT GT AC
16 161 2.07 TT GT AC 161 2.14 TT GT AC 170 2.00 CT GT AG 325 2.06 TC GT AT
17 221 2.06 TT GT AG 285 2.07 TT GT AC 221 1.91 TT GT AG 188 2.06 CT GT AT
18 497 2.05 GC GT AA 134 2.00 TT GT AG 285 1.89 TT GT AC 386 2.03 TT GT AT
19 229 1.99 TC GT AG 125 1.88 AT GT AC 452 1.84 GG GT TA 382 2.00 GT GT TT
20 293 1.90 TC GT AC 293 1.87 TC GT AC 293 1.83 TC GT AC 285 1.92 TT GT AC

The 20 most highly cleaved sites for each compound were sorted based on their relative cleavage intensity in decreasing order. The values of the cleavage intensity (percentage cleavage) has been normalised to allow for profile comparison between the analogues. In addition, the sequence of the cleavage sites are shown - the primary site in bold and their flanking nucleotides in plain text.