Fig 4. Telomerase regulates VEGF, VEGFR1, and VEGFR2 expression.

AGS (A), Caco2 (B), and HepG2/C3A (C) cells were treated with control siRNA or hTERT siRNA with or without bevacizumab, and VEGF secretion was evaluated by ELISA. (D, E) Cells were treated with BIBR-1532, costunolide, or transiently transfected with hTERT siRNA for 72h. VEGFR1 (D) and VEGFR2 (E) transcript amounts were quantified with real-time PCR. (F, G) Saos-2 cells were transiently transfected with an empty vector, hTERT-WT or hTERT-DN. VEGFR1 (F) and VEGFR2 (G) were then quantified with real-time PCR. Results were expressed as the mean ± SD from three experiments.