Table 1.

Extant and ancestral enzymes in this study and their amino acid sequence identity with hydroxynitrile lyase HbHNL and esterase SABP2.^a

Enzyme	Construct Originb	% ID to HbHNL	% ID to SABP2
HbHNL	Hevea brasiliensis	100	44
MeHNL	Manihot esculenta	76	41
HNL1	max parsimony & likelihood	79	49
HNL1-ML	maximum likelihood	75	51
HNL1-NJ	neighbor joining	79	50
EST3	max parsimony & likelihood	67	55
EST3-ML	maximum likelihood	58	60
EST3-NJ	neighbor joining	56	59
EST2	max parsimony & likelihood	48	71
EST1	max parsimony & likelihood	49	70
AtEST	Arabidopsis thaliana	47	50
RcEST	Ricinus communis	21	28
RsEST	Rauvolfia serpentina	41	56
SABP2	Nicotinia tabacum	44	100

^aSupporting information Table S1 compares the sequence identity for each enzyme pair and Figure S1 shows the aligned amino acid sequences.

^bPlant species origin for extant enzymes and methods used to derive the ancestral sequences.