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. 2017 May 9;40(1):21–30. doi: 10.3892/ijmm.2017.2980

Figure 3.

Figure 3

Overexpression of caveolin 1 (Cav1) or mitochondrial carrier homolog 2 (MTCH2) enhances the sensitivity to dihydroartemisinin (DHA). (A) G418-resistant colonies of HeLa cells transfected with empty vector (Control) or Cav1-Myc expression vector (Cav1-Myc). (B) G418-resistant colonies of HeLa cells transfected with empty vector (Control) or MTCH2-Myc expression vector (MTCH2-Myc). (C) Western blot analysis of HeLa cells stably transduced with Cav1-Myc virus (HeLa/Cav1-Myc) and HeLa cells stably transduced with empty viral vector (HeLa/Control). β-tubulin was used as loading control. (D) Western blot analysis of HeLa cells stably transduced with MTCH2-Myc virus (HeLa/MTCH2-Myc) and HeLa cells stably transduced with empty vector virus (HeLa/Control). β-tubulin was used as loading control. (E) The viability of HeLa/Cav1-Myc cells and HeLa/Control cells was measured by MTT assay after being treated with the vehicle or DHA at the indicated concentrations for 36 h. (F) The viability of HeLa/MTCH2-Myc cells and HeLa/Control cells were measured by MTT assay after being treated with the vehicle or DHA at the indicated concentrations for 36 h. Values were normalized against the vehicle control group. Data are presented as the means ± SEM of 3 independent experiments. *P<0.05, **P<0.01 compared with the vehicle control group.