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. 2017 May 9;40(1):21–30. doi: 10.3892/ijmm.2017.2980

Figure 5.

Figure 5

Caveolin 1 (Cav1) contributes to the downregulation of NAD(P)H:quinone oxidoreductase 1 (NQO1) following treatment with dihydroartemisinin (DHA). (A) HeLa cells were treated with the vehicle or DHA for 36 h and then harvested for western blot analysis to detect the protein level of NQO1 and Cav1. β-tubulin was used as a loading control. (B) Western blot analysis of HeLa/Cav1-Myc cells and HeLa/Control cells to detect the protein level of NQO1. β-tubulin was used as a loading control. (C) Western blot analysis of HeLa cells (HeLa) and Cav1 knockout cell line (Cav1-KO) to detect the protein level of NQO1. GAPDH was used as a loading control. (D-F) Quantification of the protein levels of NQO1 in panels A, B and C, respectively. Values were normalized against the vehicle group or loading control, respectively. Data are presented as the means ± SEM of 3 independent experiments. *P<0.05, **P<0.01 compared with the control group.