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. 2017 May 18;40(1):57–64. doi: 10.3892/ijmm.2017.2995

Figure 3.

Figure 3

miR-let-7g inhibits lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) expression in human aortic smooth muscle cells (ASMCs) by binding to its 3′ untranslated region (3′UTR). (A) A sequence complementary to the seed sequence of let-7g was observed in the 3′UTR of LOX-1 mRNA in the human (Hsa-) and mouse (Mmu-). (B) ASMCs were co-transfected with pmirGLO-LOX-1-3′UTR luciferase reporter plasmid and pcDNA3.1-let-7g, mutant or pcDNA3.1 plasmid. At 24 h post-transfection, luciferase assay was performed. A reporter containing not LOX-1-3′UTR but pmirGLO was used as a negative control. (C and D) ASMCs were transfected with pcDNA3.1-let-7g or its control plasmid, and then (C) the expression levels of LOX-1 mRNA and (D) LOX-1 protein were determined using real-time PCR and western blot analysis, respectively. β-actin served as the internal control. Data are presented as mean ± SD (n=3). **p<0.01 vs. the control; ##p<0.01 vs. the pcDNA3.1.