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. 2017 May 10;40(1):101–111. doi: 10.3892/ijmm.2017.2985

Figure 7.

Figure 7

TUNEL assay in the chondrocytes in growth plate ex vivo. (A) Representative images of TUNEL staining in cells. The newborn mouse metatarsals were explanted and cultured in the presence of conditioned medium of (b) BMP2 (300 ng/ml), (c) BMP2 + siPERK, (d) BMP2 + siATF4 or (e) BMP2 + siPERK + siATF4 for 5 days and were then analyzed for apoptosis by TUNEL-staining assay, (a) NC is the control. The FITC-labeled TUNEL-positive cells were imaged under a fluorescence microscope (×100 magnification). The cells with green fluorescence were recognized as apoptotic cells, and the scale bars represent 100 μm. (B) Analysis of results of cell apoptosis. Data are the means ± SD for relative apoptosis normalized to control cells for 3 independent experiments. Columns are the means of 3 separate experiments; bars represent SD. *P<0.05 as determined by Student's t-test, vs. BMP2 and BMP2 + siPERK group; BMP2 and BMP2 + siATF4 group; **P<0.01, vs. BMP2 and BMP2 + siPERK + siATF4 group. BMP2, bone morphogenetic protein 2.