Figure 4.

LC3 puncta formation is increased in the HeLa cells at 24 h.p.i. HeLa cells were transfected with plasmid pEGFP-LC3 or the empty vector (pEGFP-C3) using Lipofectamine 2000 (Invitrogen Life Technologies). Twenty-four hours later, fluorescence microscopy demonstrated the successful transduction of cells with pEGFP-LC3 and pEGFP-C3. Then, the cells were seeded in 6-well plates. (B-D) Twenty-four hours later, the cells were infected with CVB3, and (A) mock-infected cells served as the control group. LC3, light chain 3; h.p.i., hours post-inoculation; CVB3, coxsackievirus B3.