Figure 5.

Inhibition of protein kinase C β2 (PKCβ2) pathway protects INS-1 cells from advanced glycation end product (AGE)-induced apoptosis. (A) Western blot analysis was used to detect the protein expression of tribbles homolog 3 (TRB3), PKCβ2 and NADPH. β-actin was the load control of the protein blot. Optical densitometry quantified the brightness of protein bands; (B and C) TUNEL staining and FACS analysis detected apoptosis of the 48 h AGEs-treated INS-1 cells with 10 nM LY333531 or without LY333531 invention. Scale bar, 50 µm. Data are as the means ± SEM (n=3). ^*P<0.05, ^**P<0.01 and ^***P<0.001, compared with control or siNC group; ^#P<0.05, compared with the AGEs group.