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. 2017 May 10;40(1):47–56. doi: 10.3892/ijmm.2017.2984

Figure 6.

Figure 6

Ubiquitin-specific protease 13 (USP13) upregulates vinculin protein levels but not the mRNA level. (A) RT-qPCR analysis of the relative mRNA level of vinculin with the expression of USP13 and normalized to that of β-actin. Data are the means ± standard error of the mean (SEM) from 3 independent experiments and analyzed by the Student's t-test. (B) Western blot analysis of vinculin protein levels with the expression of USP13. Three independent experiments were performed, and representative results are shown. (C) The vinculin protein levels in the panel were measured and represented in the same graph, β-actin was used for normalization. *P<0.05 by one-way ANOVA. Data are the means ± SEM of triplicates from 3 independent experiments.