Table I.
Summary of the proteins identified by LC-MS/MS.
| Master no.a | Accession no. | Protein name | Theoretical
|
Protein score | Protein matched name peptides | Protein sqeCover (%) | Avg ratioa | P-valueb | |
|---|---|---|---|---|---|---|---|---|---|
| Size (Da) | pI | ||||||||
| 1/I | P18206 | Vinculin | 116,722.62 | 5.90 | 5,615.65 | 81 | 84.02 | 1.23/1.21 | 0.008/0.010 |
| 2 | P52888 | Thimet Oligopeptidase (THOP1) | 78,840.00 | 5.87 | 1,693.12 | 13 | 37.48 | 1.21 | 0.008 |
| 3 | G5E9W3 | Cleavage and polyadenylation specific factor 3 (CPSF3) | 77,486.24 | 5.42 | 1,053.86 | 19 | 38.31 | 1.16 | 0.008 |
| 4 | Q5T6W5 | SH3 domain containing RING finger protein 3 | 93,973.91 | 9.99 | 1,580.02 | 11 | 25.81 | 1.15 | 0.019 |
| 5/IIc | B3GQS7 | 1.32/1.31 | 0.017/0.011 | ||||||
| 6/IIIc | F5GWR2 | 1.16/1.15 | 0.02/0.024 | ||||||
| 7 | Q9BQA1 | Methylosome protein 50 (WDR77) | 36,724.50 | 4.95 | 2,015.87 | 20 | 61.25 | 1.17 | 0.013 |
| 8 | F8W9D6 | Adenylosuccinate synthetase (ADSS) | 48,338.13 | 6.37 12,416.72 | 39 | 89.20 | −1.16 | 0.016 | |
| 9 | E7EW96 | Annexin | 38,714.27 | 6.86 | 5,310.28 | 16 | 79.14 | −1.2 | 0.014 |
| 10 | P17987 | T complex protein 1 subunit α | 43,885.80 | 7.67 | 751.85 | 17 | 44.11 | −1.15 | 0.004 |
| 11 | Q6P6D7 | Phosphoglycerate mutase (PGAM) | 28,776.89 | 6.46 39,293.07 | 13 | 55.12 | −1.23 | 0.010 | |
a
1, 5, 6 indicate the spots following USP13 overexpression compared with EGFP, and I, II, III indicate the spots following USP13 overexpression compared with USP13 C345S;
b
The average ratio of gel spots in 2D-DIGE, P-value was determined by the Student's t-test;
c
failure to identify. LC-MS, liquid chromatography-mass spectroscopy; 2D-DIGE, two-dimensional difference gel electrophoresis; USP13, ubiquitin-specific protease 13.