Table II.
Primary antibodies.
| Primary antibodies (Cat. no.) | Companies | Dilute solution | Dilution rate |
|---|---|---|---|
| Rabbit anti-GAPDH (ab181602) | Abcam | 5% non-fat milk | 1:10,000 |
| Mouse anti-CD31 (ab24590) | Abcam | 5% non-fat milk | 1:500 |
| Rabbit anti-VE-cadherin (#2500) | Cell Signaling Technology | 5% BSA | 1:500 |
| Rabbit anti-α-SMA (ab32575) | Abcam | 5% non-fat milk | 1:5,000 |
| Rabbit anti-FSP-1 (ab41532) | Abcam | 5% BSA | 1:1,000 |
| Rabbit anti-Notch1 (ab52627) | Abcam | 5% non-fat milk | 1:1,000 |
| Rabbit anti-Slug (#9585) | Cell Signaling Technology | 5% BSA | 1:1,000 |
| Rabbit anti-Snail (#3879) | Cell Signaling Technology | 5% BSA | 1:1,000 |
| Rabbit anti-Smad2/3 (bs-3484R) | Bioss | 5% non-fat milk | 1:1,000 |
| Rabbit anti-p-Smad2/3 (bs-8853R) | Bioss | 5% non-fat milk | 1:1,000 |
| Rabbit anti-RUNX3 (bs-0378R) | Bioss | 5% non-fat milk | 1:1,000 |
| Mouse anti-TGF-β2 (ab167655) | Abcam | 5% BSA | 1:1,000 |
| Rabbit anti-VEGFR1 (bs-0170R) | Bioss | 5% non-fat milk | 1:1,000 |
α-SMA, α-smooth muscle actin; FSP-1, fibroblast-specific protein-1; RUNX3, runt-related transcription factor 3; TGF-β2, transforming growth factor-β2; VEGFR1, vascular endothelial growth factor receptor 1.