Figure 3. Yap is required for SMG ductal epithelial patterning.
(A) IF analysis of Yap (green) and Krt14 (K14, white) in E15.5 WT and Yap-cnull SMGs. Scale = 10 µm. (B–G) E13.5 WT and Yap-cnull SMGs were dissected and cultured for 24 hr ex vivo and then examined by microscopy. (B) Phase-contrast images of E13.5 WT and Yap-cnull SMGs at the time of dissection and 24 hr after culture. The same SMGs (each column is one SMG) were analyzed by IF for (C) Krt14 (K14, magenta), (D) Krt5 (K5, green), (E) Krt19 (K19, red), (F) TuJ1 (yellow), and (G) F-actin (white, Phalloidin). DAPI was used to mark the nuclei (blue). Scale = 100 μm. All images represent observations made from a minimum of three biological repeats.