Appendix 2—table 1.

Metabolic convergence between squid native V. fischeri ES114 and squid-evolved binK1. The net changes in metabolic activity (as indicated by change in absorption of the Biolog tetrazolium redox dye) are shown for each V. fischeri strain after 48 hr of exposure to each substrate. Only substrates which induced significant (FDR < 0.05) differences across strains are listed. Metabolic changes in each strain relative to wild-type MJ11 binK⁺ are colored to indicate relatively increased or decreased activity. Of the 190 substrates tested, 44 substrates yielded significant differences across strain, including 39 which indicate congruent metabolic responses by ES114 and binK1 (Exact binomial test, p=1.405e-7).

DOI: http://dx.doi.org/10.7554/eLife.24414.031

		Metabolic activity (∆A550 over 48 hr)				Metabolic activity change relative to remS+ MJ11
Well	Substrate	remS+	remS1	∆remS	ES114	remS+	remS1	∆remS	ES114	Convergence
H11	Phenylethylamine	0.012	0.568	0.458	0.667	0.000	46.54	37.33	54.85	+
H07	Glucuronamide	0.017	0.523	0.564	0.558	0.000	30.20	32.69	32.30	+
G10	Methyl pyruvate	0.019	0.677	0.462	0.639	0.000	33.70	22.70	31.78	+
H08	Pyruvic acid	0.013	0.187	0.276	0.395	0.000	13.09	19.76	28.71	+
E01	L-Glutamine	0.026	0.620	0.125	0.665	0.000	22.82	3.78	24.54	+
F03	m-Inositol	0.044	0.724	0.726	0.671	0.000	15.50	15.54	14.28	+
E02	m-Tartaric acid	0.026	0.424	0.338	0.451	0.000	15.10	11.84	16.13	+
D02	D-Aspartic acid	0.040	0.459	0.363	0.735	0.000	10.56	8.16	17.55	+
A12	Dulcitol	0.030	0.402	0.091	0.608	0.000	12.61	2.07	19.63	+
G03	L-Serine	0.032	0.467	0.235	0.360	0.000	13.66	6.39	10.29	+
H02	p-Hydroxy phenyl acetic acid	0.027	0.063	0.037	0.636	0.000	1.27	0.36	22.11	+
B06	D-Gluconic acid	0.048	0.300	0.324	0.628	0.000	5.24	5.75	12.06	+
B09	L-Lactic acid	0.029	0.068	0.040	0.647	0.000	1.30	0.37	20.93	+
E09	Adonitol	0.026	0.358	0.197	0.085	0.000	12.88	6.63	2.28	+
H01	Glycyl-L-proline	0.039	0.206	0.258	0.443	0.000	4.32	5.65	10.44	+
C05	Tween 20	0.025	0.065	0.001	0.517	0.000	1.60	−0.95	19.54	+
E08	β-Methyl-D-glucoside	0.004	0.024	0.022	0.021	0.000	5.66	5.10	4.85	+
G05	L-Alanine	0.056	0.322	0.295	0.355	0.000	4.74	4.25	5.33	+
B11	D-Mannitol	0.018	0.177	0.085	0.034	0.000	8.64	3.64	0.88	+
H09	L-Galactonic acid—Lactone	0.089	0.275	0.379	0.450	0.000	2.10	3.27	4.06	+
F04	D-Threonine	0.017	0.126	0.044	0.041	0.000	6.37	1.55	1.37	+
D01	L-Asparagine	0.026	0.069	0.080	0.129	0.000	1.61	2.02	3.86	+
H06	L-Lyxose	0.088	0.051	0.036	0.481	0.000	−0.42	−0.59	4.48	-
F8	Mucic acid	0.026	0.072	0.044	0.035	0.000	1.78	0.68	0.36	+
C12	Thymidine	0.071	0.168	0.116	0.052	0.000	1.36	0.63	−0.27	-
G11	D-Malic acid	0.028	0.062	0.036	0.029	0.000	1.21	0.31	0.05	+
F06	Bromo succinic acid	0.033	0.061	0.037	0.035	0.000	0.82	0.11	0.04	+
A10	D-Trehalose	0.031	0.045	0.038	0.034	0.000	0.45	0.21	0.08	+
D06	α-Keto-glutaric acid	0.045	0.074	0.043	0.042	0.000	0.65	−0.04	−0.08	-
F9	Glycolic acid	0.039	0.062	0.032	0.040	0.000	0.61	−0.17	0.04	+
C11	D-melibiose	0.028	0.043	−0.006	0.053	0.000	0.53	−1.20	0.90	+
D10	Lactulose	0.045	0.057	0.046	0.035	0.000	0.27	0.02	−0.20	-
A10	Laminarin	0.678	0.546	0.674	0.798	0.000	−0.20	−0.01	0.18	-
E06	2-Hydroxy benzoic acid	0.089	0.070	0.080	0.093	0.000	−0.21	−0.10	0.04	+
A03	α-Cyclodextrin	0.191	0.122	0.089	0.158	0.000	−0.36	−0.54	−0.17	+
H07	D,L-Octopamine	0.200	0.111	0.067	0.186	0.000	−0.45	−0.66	−0.07	+
F07	D-Ribono-1,4-lactone	0.198	0.085	0.070	0.162	0.000	−0.57	−0.65	−0.18	+
D07	Turanose	0.188	0.065	0.060	0.137	0.000	−0.66	−0.68	−0.27	+
E02	Caproic acid	0.241	0.101	0.007	0.215	0.000	−0.58	−0.97	−0.11	+
G10	L-Leucine	0.214	0.051	0.075	0.135	0.000	−0.76	−0.65	−0.37	+
G02	L-Alaninamide	0.164	0.065	0.047	0.045	0.000	−0.60	−0.72	−0.72	+
G02	Tricarballylic acid	0.029	0.018	−0.008	0.006	0.000	−0.37	−1.27	−0.80	+
C10	α-Methyl-D-mannoside	0.183	0.004	0.024	0.075	0.000	−0.98	−0.87	−0.59	+
D08	α-Methyl-D- Galactoside	−0.011	0.018	0.008	0.000	0.000	−2.56	−1.68	−0.99	+