Table 1.
Strains and plasmids used in this study.
| Strain name | Description* | Reference/source |
|---|---|---|
| Vibrio fischeri strains† | ||
| ES114 | Isolated from Euprymna scolopes | (Boettcher and Ruby, 1990) |
| MJ11 | Isolated from Monocentris japonica light-organ | (Haygood et al., 1984) |
| EM17 | Isolated from Euprymna morseii light-organ | (Ruby and Lee, 1998) |
| H905 | Isolated from Hawaiian plankton | (Lee and Ruby, 1992) |
| WH1 | Isolated from Massachusetts plankton | (Lee, 1994) |
| RF1A4 | MJ11 ∆binK::ermB; EmR | This study |
| RF1A5 | MJ11 ∆sypK::aphA1; KmR | This study |
| RF1A6 | MJ11 ∆binK::ermB ∆sypK::aphA1; EmR KmR | This study |
| RF1A7 | MJ11 binK1 ∆sypK::aphA1; KmR | This study |
| MJ11EP2-3-2 | MJ11 binK4 | This study |
| MJ11EP2-3-3 | MJ11 binK4 | This study |
| MJ11EP2-3-4 | MJ11 binK4 | This study |
| MJ11EP2-3-5 | MJ11 binK4 | This study |
| MJ11EP2-3-6 | MJ11 binK4 | This study |
| MJ11EP2-3-7 | MJ11 binK4 | This study |
| MJ11EP2-3-8 | MJ11 binK4 | This study |
| MJ11EP15-3-1 | MJ11 binK4 | This study |
| MJ11EP15-3-3 | MJ11 binK4 | This study |
| MJ11EP15-3-4 | MJ11 binK4 | This study |
| MJ11EP15-3-7 | MJ11 binK4 | This study |
| MJ11EP15-3-8 | MJ11 binK4 | This study |
| MJ11EP2-4-1 | MJ11 binK1 | This study |
| MJ11EP2-4-3 | MJ11 binK1 | This study |
| MJ11EP2-4-4 | MJ11 binK1 | This study |
| MJ11EP2-4-5 | MJ11 binK1 | This study |
| MJ11EP2-4-6 | MJ11 binK1 | This study |
| MJ11EP15-4-1 | MJ11 binK1 tadC1G593T | (Schuster et al., 2010) |
| MJ11EP15-4-6 | MJ11 binK1 | This study |
| MJ11EP15-4-7 | MJ11 binK1 | This study |
| MJ11EP15-4-8 | MJ11 binK1 | This study |
| MJ11EP2-5-2 | MJ11 binK3 | This study |
| MJ11EP2-5-3 | MJ11 binK3 | This study |
| MJ11EP2-5-4 | MJ11 binK3 | This study |
| MJ11EP2-5-5 | MJ11 binK3 | This study |
| MJ11EP2-5-6 | MJ11 binK3 | This study |
| MJ11EP15-5-2 | MJ11 binK4 | This study |
| MJ11EP15-5-3 | MJ11 binK3 | This study |
| MJ11EP15-5-4 | MJ11 binK3 | This study |
| MJ11EP15-5-5 | MJ11 binK3 | This study |
| MJ11EP2-6-1 | MJ11 binK2 | This study |
| MJ11EP15-6-1 | MJ11 binK2 | (Schuster et al., 2010) |
| MJ11EP15-6-2 | MJ11 binK2 | This study |
| MJ11EP15-6-3 | MJ11 binK2 | This study |
| MJ11EP15-6-4 | MJ11 binK2 | This study |
| MJ11EP15-6-5 | MJ11 binK2 | This study |
| MJ11CE4-1 | MJ11 fliAG80D | This study |
| MJ11CE5-1 | MJ11 fliP∆476 | This study |
| Strain name | Description* | Reference/source |
| Escherichia coli strains | ||
| DH5α | F−
recA1 endA1 hsdR17 supE44 thi-1 gyrA96
relA1Δ (argF-lacZYA) U169φ 80lacZΔM15λ − |
Gibco-BRL |
| DH5αλpir |
supE44 ΔlacU169 (ϕlacZΔM15) recA1 endA1 hsdR17
thi-1 gyrA96 relA1; λpir phage lysogen |
(Kolter and Helinski, 1978) |
| CC118λpir | Δ(arg-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE
rpoB argE(Am) recA1, lysogenized with λ pir dam dcm |
(Martín-Mora et al., 2016) |
| NEB 10-beta | Δ(ara-leu)7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1
relA1 endA1 nupG rpsL (SmR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) |
New England Biolabs, Ipswich, MA |
| TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1
araD139 Δ(ara-leu)7697 galU galK rpsL (SmR) endA1 nupG |
Invitrogen, Carlsbad, CA |
| Plasmids | ||
| pCR2.1-TOPO | Commercial cloning vector; ApR KmR | Invitrogen, Carlsbad, CA |
| pVSV105 | Mobilizable vector; ChR | (Dunn et al., 2006) |
| pRAD2E1 | pVSV105 carrying wild-type binK; ChR | This study |
| pRF2A2 | pVSV105 carrying binK1; ChR | This study |
| pCLD48 | pVSV105 carrying ES114 sypE; ChR | (Hussa et al., 2008) |
| pRF2A3 | pVSV105 carrying MJ11 binA; ChR | This study |
| pVSV104 | Mobilizable vector; KmR | (Stabb and Ruby, 2002) |
| pRF2A1 | pVSV104 carrying sypE; KmR | This study |
| pRF2A4 | pVSV104 carrying binA; KmR | This study |
| pKV111 | Mobilizable vector containing gfp; ChR | (Nyholm et al., 2000) |
| pRF2B7 | pCR2.1-TOPO containing MJ11 ∆sypK::aph1 SOE fragment; KmR | This study |
| pVSV103 | Mobilizable vector containing lacZ; KmR | (Dunn et al., 2006) |
| pCAW7B1 | pVSV103 containing lacZ∆147–1080 bp; KmR | This study |
*ApR, ampicillin resistance; ChR, chloramphenicol resistance; EmR, erythromycin resistance; KmR, kanamycin resistance;SmR streptomycin resistance.
†Experimentally evolved strains are designated ‘MJ11EP#-#-#’, where the first and second numbers after the ‘P’ designates the squid passage and population from which the strain was isolated, and the third number designates isolate number; strains derived from evolution in culture are designated ‘MJ11CE’.