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. 2017 Jan 18;15(7):824–836. doi: 10.1111/pbi.12678

Figure 5.

Figure 5

Stable FIT2 expression increases oil content in Arabidopsis seeds. (a) Representative confocal images of LDs in wild‐type and FIT2 transgenic (i.e. FIT2‐OE‐C6 line) Arabidopsis seeds. LDs were stained with Nile red (false‐coloured yellow). Bar = 5 μm. (b) Mature seeds from wild‐type and FIT2 transgenic Arabidopsis plants. Bar = 0.5 mm. (c) to (f) Seed size and seed oil content of wild‐type and FIT2 transgenic Arabidopsis. Averages and SDs were calculated from three biological replicates. Seeds were collected from Arabidopsis plants grown under the same conditions. Asterisks indicate significant difference at P ≤ 0.05 determined by Student's t‐test. (c) Weight of 100 dry seeds. (d) Oil content in dry seeds on a weight basis measured by NMR. (e) Oil content per seed on a fatty acid basis quantified by GC‐FID. (f) Fatty acid composition in mature seeds.