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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Mol Cancer Res. 2017 Feb 27;15(6):696–707. doi: 10.1158/1541-7786.MCR-16-0361

Figure 2. HuR-deficient cells induce more cell death.

Figure 2

A, Cells were stained with Trypan blue and number of dead cells was counted after 24, 48 and 72 hours for one clone depicting each mutation. Graphs are average of three individual experiments. * p<0.003; ** p<0.004. B and C, Cell death was also assessed by cleaved caspase 3-activity after 24 hours of cell seeding via FACS. Graphs are average of three individual experiments. * p<0.0004. D and E, Long-term cell survival assay was assessed by colony formation soft agar assay. Graphs are average of two individual experiments. * p<0.003. F, Cell kinetics was measured by pulse chasing the cells by BrdU followed by FACS. Graphs are average of two individual experiments.