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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Bone Miner Res. 2017 Mar 27;32(6):1343–1353. doi: 10.1002/jbmr.3114

Figure 3.

Figure 3

Disruption of the gut microbiota with antibiotics did not alter total bacterial load, but had dramatic effects on gut microbiota composition and bacterial diversity, and immune cell count. (A) The relative composition of bacterial phyla shifted from a Bacteroidetes dominated phyla in untreated mice to one dominated by Proteobacteria in ΔMicrobiota mice (n=6/group). (B) Proteobacteria is enriched in ΔMicrobiota mice, especially in TLR5KO ΔMicrobiota mice (Bonferroni correction). (C) Bacteroidetes dominates gut microbiota composition in untreated WT and TLR5KO. (D) Total bacterial load was unaffected by antibiotic treatment. (E) Bacterial diversity was dramatically reduced in ΔMicrobiota mice. (F) The percentage of splenic CD20+ B cells was reduced in ΔMicrobiota mice and untreated TLR5KO mice (n=3/group). (G) The percentage of splenic CD3+ T cells in the spleen was reduced in ΔMicrobiota mice and untreated TLR5KO mice (n=3/group). Solid colored lines on dot plots represent mean. * p < 0.05 after adjusting for multiple comparisons