Table 1.
Micro-computed tomography measures of cancellous and cortical bone and whole bone mechanical testing measures after adjustments for body mass are shown.
| Wild Type | TLR5KO | ||||
|---|---|---|---|---|---|
| Body Mass Adjusted Measure | Untreated | ΔMicrobiota | Untreated | ΔMicrobiota | |
| Proximal Tibia | Bone Volume Fraction | 0.15 ± 0.02 | 0.15 ± 0.03 | 0.14 ± 0.01 | 0.14 ± 0.01 |
| Trabecular Thickness (μm) | 0.045 ± 0.002 | 0.043 ± 0.005 | 0.041 ± 0.003 a | 0.038 ± 0.002$ | |
| Trabecular Separation (μm) | 0.197 ± 0.014 | 0.192 ± 0.008 | 0.187 ± 0.007 | 0.178 ± 0.007$ | |
| Cortical TMD (mg HA/cm3) | 999 ± 10 | 979 ± 8* | 999 ± 13 | 980 ± 7# | |
| Growth Plate Thickness (μm) | 558 ± 46 | 562 ± 41 | 550 ± 54 | 528 ± 21 | |
| Femoral Diaphysis | Cortical Area (mm2) | 0.87 ± 0.07 | 0.81 ± 0.09* | 0.94 ± 0.07 | 0.69 ± 0.04# |
| Marrow Area (mm2) | 1.05 ± 0.06 | 1.17 ± 0.10* | 1.17 ± 0.15 | 1.00 ± 0.08# | |
| Total Area (mm2) | 1.93 ± 0.12 | 1.98 ± 0.17 | 2.12 ± 0.17 a | 1.68 ± 0.08#,$ | |
| Cortical Thickness (μm) | 210 ± 10 | 189 ± 15* | 216 ± 13 | 172 ± 12#,$ | |
| Moment of Inertia (mm4) | 0.15 ± 0.02 | 0.14 ± 0.02 | 0.17 ± 0.03 a | 0.10 ± 0.01#,$ | |
| Moment of Inertia/c (mm3) | 0.22 ± 0.03 | 0.22 ± 0.03 | 0.25 ± 0.03 | 0.17 ± 0.01#,$ | |
| Whole Femur | Length (mm) | 16.04 ± 0.18 | 16.13 ± 0.12 | 15.80 ± 0.24a | 15.71 ± 0.12$ |
| Peak Bending Moment (N* mm) | 40.79 ± 2.71 | 37.18 ± 2.35* | 38.75 ± 2.37 | 30.22 ± 1.76#,$ | |
| Bending Stiffness (N/mm) | 193 ± 26 | 177 ± 21 | 175 ± 33 | 152 ± 7 | |
| Post Yield Displacement (mm) | 0.20 ± 0.17 | 0.35 ± 0.09 | 0.29 ± 0.11 | 0.42 ± 0.07 | |
Values are mean ± SD. TMD=Tissue mineral density
*
WT-ΔMicrobiota v. WT-untreated, p<0.05
#
TLR5KO-ΔMicrobiota v. TLR5KO-untreated, p<0.05
a
TL5KO-untreated v. WT-untreated, p<0.05
$
TLR5KO-ΔMicrobiota v. WT-ΔMicrobiota, p<0.05