Figure 3.

Effects of stilbene analogues and dexamethasone (DEX) on the AP‐1 pathway. (A) Representative blot and (B) quantification of total c‐Jun protein expression in A549 cells treated with various concentrations of isorhapontigenin (ISO) or resveratrol (RES) (1–100 μM) or 1 μM DEX for 1 h before stimulation with 1 ng·mL⁻¹ IL‐1β for 60 min. IL‐1β increased the expression of c‐Jun where * represents P < 0.05 for differences from non‐stimulated cells (NS). This stimulation was reduced by stilbene analogues and DEX (n = 5), where *indicates P < 0.05 for differences from IL‐1β stimulated cells by ANOVA followed by Bonferroni correction. (C) c‐Jun mRNA levels were significantly increased after IL‐1β stimulation (P < 0.05) for 30 min, and this was significantly attenuated by ISO (P < 0.05) and to a lesser extent by RES and DEX (n = 5) where * represents P < 0.05 as measured by ANOVA with Bonferroni correction for differences from IL‐1β stimulation. (D) Representative blot and quantification (E) of total c‐Fos protein expression in A549 cells treated with 100 μM stilbene analogue or 1 μM DEX for 1 h before stimulation with 1 ng·mL⁻¹ IL‐1β for 60 min. IL‐1β increased the levels of c‐Fos, which was enhanced by stilbene analogues but dexamethasone had no effect (n = 4). Data are mean ± SEM, and * represents P < 0.05 as measured by ANOVA with Bonferroni correction.