Figure 4.
Effects of treatment with DBHB (1.6 mmol·kg−1·day−1) on NLRP3 inflammasome activation in spinal cord of mice exposed to SCI. Samples of tissue (5 mm) centred on the injury epicentre or equivalent area in sham‐operated mice were manually dissected for measurements. Western blotting results and corresponding quantification (A) of caspase‐1, pro‐caspase‐1 and NLRP3 are shown. Graphs show the levels of IL‐1β (B) and IL‐18 (C) and activity of MPO (D). Data are presented as the mean ± SD of eight mice per group. *P < 0.05, significantly different from sham‐operated mice; #P < 0.05, significantly different fromSCI mice.