Figure 1.

The use of automated video tracking to simultaneously assess multiple phenotypes in larval zebrafish. Panel (A) shows a 96‐well holding plate to administer several compounds to larval zebrafish. Fish behaviours are recorded by an overhead camera, and images are processed through tracking software. Swim traces garnered from the tracking software allow the researcher to assess the effects of the compounds administered. Panel (B) shows an example of a swim trace in which the larval zebrafish stay close to the walls (wall‐hugging behaviour). Panel (C) shows an example of the opposite swim pattern in which the larval zebrafish actively explore their environment, including the centre of the tank.