Figure 5.

ZP1609 does not affect mitochondrial calcium retention capacity. (A) Original traces showing Calcium Green 5 N fluorescence of control mitochondria (SSM and IFM) and mitochondria treated with 1 μM ZP1609 in the absence (left panel) or presence (right panel) of cyclosporin A (CsA). CaCl₂ (5 μM) was added every third minute until a sudden increase in Calcium Green 5 N fluorescence occurred reflecting MPTP opening. (B, C) The concentration of CaCl₂ at which MPTP opening takes place was calculated in SSM and IFM in the absence or presence of CsA in mitochondria incubated under control conditions (con) or with 1 μM ZP1609 (ZP, B) or 10 μM ZP1609 (C), respectively, * P < 0.05, significantly different as indicated.