Figure 1.

Schematic of the Single Cell-Based Digital Droplet PCR System

Cells from the target population were encapsulated into droplets at a concentration of one cell/droplet with the PCR mixture including primers and probes. After single-cell encapsulation, cell lysis and amplification of the target sequence were performed within the droplets. The number of droplets with a fluorescent signal indicates the number of cells carrying the target or reference gene. sc-ddPCR, single cell-based digital droplet PCR.