Fig. 2.

RPE from donors with AMD show reduced glycolytic function and resistance to oxidative inactivation. (A,B) Trace shows extracellular acidification rate (ECAR) normalized to baseline for cells from non-diseased and AMD donors with no treatment (A, No AMD n=9; AMD n=9) and following 24 h incubation with 500 µM hydrogen peroxide (B, No AMD n=7; AMD n=6). Arrows indicate injection of glucose (1), oligomycin (2), and 2-deoxyglucose (2-DG,3). (C,D,E) Parameters of glycolytic function calculated from data shown in A or B. Probability values for significant differences, as determined by t-test comparing No AMD with AMD (C) or paired t-test comparing glycolytic function with and without oxidation for individual samples (D,E), is provided on the graphs. See Supplement Fig. 1A for the method of calculation. All data are mean (± SEM). (* denotes p<0.05).