Fig. 3.

RPE from donors with AMD show reduced mitochondrial function. (A,B) Trace from an XF⁹⁶ Extracellular Flux Analyzer shows the oxygen consumption rate (OCR) normalized to baseline for cells from non-diseased and AMD donors with no treatment (A, No AMD n=14; AMD n=19) and following 24 h incubation with 500 µM hydrogen peroxide (B, No AMD n=10; AMD n=15). Arrows indicate injection of oligomycin (1), FCCP (2) and antimycin and rotenone (3) to perturb mitochondrial function. (C,D,E) Parameters of mitochondrial function were calculated from data shown in A or B. Probability values for significant differences, as determined by t-test comparing No AMD with AMD (C) or paired t-test comparing values with and without oxidation for individual samples (D,E), is provided on the graphs. Bas Res=basal respiration; Max Res=maximal respiration; Sp Cap=spare capacity. See Supplement Fig. 1B for the method of calculation. All data are mean (± SEM). (* denotes p<0.05).