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. 2017 Jun 9;7:3174. doi: 10.1038/s41598-017-03634-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic oxidation process of the Heme and Metheme, respectively. (a) and (d) Fluorescent spectra of the Heme and Metheme before and after the oxidation. (b) and (e) Time dependent variation of the fluorescent peak intensities during the oxidation process. (c) and (f) Comparison experiments via the Hemoglobin specimen from normal person and SCD patient, which are provided by Ruijin Hospital.