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. 2017 Jun 9;7:3193. doi: 10.1038/s41598-017-03506-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA sequencing-based analysis of differentially expressed genes (DEG) by EIN3 and AKIN10. (a,b) Comparative analysis of DEG up-regulated by EIN3 and down-regulated by AKIN10 (a) and those down-regulated by EIN3 and up-regulated by AKIN10 (b). (c,d) Expression of genes up-regulated by EIN3 and down-regulated by AKIN10 (c) and those down-regulated by EIN3 and up-regulated by AKIN10 (d) were measured using Arabidopsis protoplasts in combination of EIN3 and AKIN10 using RT-qPCR. (e,f) Comparative analysis of DEG up-regulated by both EIN3 and AKIN10 (e) and those down-regulated by both EIN3 and AKIN10 (f). (g) Meta-analysis of DEG up-regulated by both EIN3 and AKIN10 with those by hypoxia. (h) Expression of genes up-regulated by both EIN3 and AKIN10 was measured in combination of EIN3 and AKIN10 using RT-qPCR. (c,d,h) Values are means of triplicates with standard error bars (*p < 0.001; **p < 0.01; ***p < 0.05). All experiments were repeated with consistent results.