Restriction digests and Southern hybridization of phage DNA. (a) HindIII and XbaI double digest of phage DNA. Lanes: 1, λ, HindIII DIG-labeled marker (Roche); 2, φC2; 3, φC5; 4, φC6; 5, φC8. (b) Southern hybridization of gel shown in panel a with DIG-labeled φC6 genomic probe (25 ng ml−1).