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. 2005 Feb;71(2):1079–1083. doi: 10.1128/AEM.71.2.1079-1083.2005

FIG. 3.

FIG. 3.

Determining the site of chromosomal prophage integration through Southern hybridization of DIG-labeled φC2 to DNA of lysogen(s). (a) Agarose gel of HindIII-digested DNA. Lanes: 1, λ HindIII DIG-labeled marker (Roche); 2, CD839; 3, CD839C2; 4, φC2. (b) A Southern blot of the gel shown in panel a with a φC2 genome probe (25 ng ml−1) revealed differences between the banding patterns of CD839C2 and φC2 in the 1.9- to 2-kb region. (c) Southern hybridization of the 1.9-kb HindIII fragment (2.5 ng ml−1) to DNA of lysogens CD839C2 and CD843C4 resulted in new bands. Lanes: 1, CD839C2; 2, CD843C2; 3, φC2.