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. 2017 Jun 9;7:3209. doi: 10.1038/s41598-017-03319-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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APN attenuates H₂O₂-induced growth inhibition in C2C12 cells. Cells were treated with various concentrations of APN for 24 h (A) or pretreated with 30 μg/mL APN for 24 h and then incubated with or without 5 mM H₂O₂ for 1 h (D). Dose-dependent (B) and time-dependent (C) inhibition of cell growth by H₂O₂ was evaluated using a Cell Counting Kit-8. The data are expressed as percentages of the mean values ± SEM of experiments performed in triplicate (*p < 0.05, **p < 0.01, ***p < 0.001).