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. 2017 Jun 9;7:3136. doi: 10.1038/s41598-017-03475-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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COUP-TFII expression decreases during myogenic differentiation in C2C12 mouse myoblast cells. (A) Western blot analyses of COUP-TFII protein expression during myogenic differentiation in C2C12 cells. Relative COUP-TFII protein level was quantified by band density and normalized to GAPDH as shown in bottom panel. (B) Quantitative RT-PCR examination of Myogenin (MRF in early differentiation stage) and MRF4 (MRF in terminal differentiated myotube) mRNA expression pattern C2C12 cells were placed in the differentiation medium (DM, 2% horse serum) and incubated for the indicated time points. Day 0, serum withdraws in confluent culture cells as the initiation of myogenesis; medium was changed every other day. Data are shown as mean ± SEM. One-way ANOVA followed by Bonferroni’s Multiple Comparison Test; *p < 0.05, **p < 0.01, ***p < 0.001, compared to day 0.