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. 2017 Jun 9;7:3158. doi: 10.1038/s41598-017-03230-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Suppression of autophagy by down-regulated TRPC5 increases sensitivity to ADM in vivo. (A) Female nude mice were inoculated with MCF-7 or MDA-MB 231 cells transfected with control or TRPC5 shRNA lentiviral particles and treated with ADM (6 mg/kg) when the tumors reached ~100 mm³ (n = 5 in each group). Autophagy in tumor samples were assayed by LC3 stain. Scale bar: 100 μm. (B and C) Representative images and summary data from immunohistochemical staining of TRPC5 and LC3 in paired pre- and post-chemotherapy breast cancer tissue from patients showing elevated TRPC5 or LC3 expression (n = 31). Scale bar: 100 μm. (D) Pearson correction of TRPC5 expression with LC3 (n = 31). Data were analyzed using Pearson correlation test. Values are mean ± SEM *p < 0.05, compared to control shRNA, ^#P < 0.05, compared to TRPC5 shRNA.