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. 2017 Jun 9;7:3132. doi: 10.1038/s41598-017-03440-2

Figure 4.

Figure 4

Microstructural and ultramicroscopic structure changes of M. aeruginosa exposure to 4 μg/mL compound 1 and DBL for 72 h. Light micrographs of Microcystis cells: (A) Normal M. aeruginosa cells, scale bar = 20 μm; (B) M. aeruginosa cells exposed to compound 1, scale bar = 20 μm; (C) M. aeruginosa cells exposed to DBL, the arrows in B and C pointed to the shrinkage and perforation of cell membrane, scale bar = 20 μm; SEM of Microcystis cells: (D) Control M. aeruginosa cells present normal shape, scale bar = 1 μm; (E) M. aeruginosa cells exposed to compound 1, scale bar = 1 μm; (F) M. aeruginosa cells exposed to DBL, scale bar = 0.5 μm; TEM of Microcystis cells: (G) Control M. aeruginosa cells present normal shape and structure, CW: cell wall, V: vesicles, N: nucleus, scale bar = 1.0 μm; (H) M. aeruginosa cells exposed to compound 1, scale bar = 2.0 μm (I) M. aeruginosa cells exposed to DBL, scale bar = 2.0 μm. Experiments were performed in triplicate.