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. 2017 Jun 9;7:3142. doi: 10.1038/s41598-017-03252-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo optical imaging of βG activity by FDGlcU. (A) Serial whole-body images at indicated time points after oral gavage with DDW (top), FDGlcU (middle), and fluorescein (bottom). Green arrows indicate intestine and red arrows indicate bladder. (B) Total flux in abdomens of mice gavaged with FDGlcU (●) (n = 5) or fluorescein (○) (n = 5). (C) The regions of interest in different organs and tissues after oral administration of FDGlcU (n = 3) or fluorescein (n = 3) for 3 hours were analyzed with Living Image software. Error bars indicate SEM. NS, no significant difference. *P value < 0.05. **P value < 0.01. ***P value < 0.001.