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Early in the infection model, C. jejuni cells aggregated at certain positions on A. polyphaga cell walls (A and B), and after some time, live bacterial cells were observed in amoebic vacuoles (C and D). Subculturing of culture-negative samples together with fresh amoebae at 37°C resulted in lysis of the amoebae, after which live C. jejuni cells could be retrieved (E).
