Table 1:
Metrics of the SMARTdenovo S288C assemblies before and after polishing with Nanopolish using R7 reads. The Nanopore 2D reads were aligned to the most continuous SMARTdenovo assembly. The alignment was given as input to Nanopolish to correct assembly errors. Metrics were obtained by aligning the pre-polishing and post-polishing version of the assembly to the reference genome using Quast.
SMARTdenovo pre-polishing | SMARTdenovo post-polishing | |
---|---|---|
# contigs | 26 | 26 |
Cumulative size | 12 018 244 | 12 204 373 |
N50 | 771 149 | 782 423 |
N90 | 238 808 | 242 444 |
L50 | 7 | 7 |
L90 | 16 | 16 |
# mismatches | 6970 | 1930 |
# insertions | 7735 | 7707 |
# deletions | 128 050 | 17 445 |
# deletions in homopolymers | 79 152 | 6869 |
# genes | 6251 + 24 partial | 6273 + 15 partial |
# genes without indels | 429 | 2590 |