Fig. 2.

Coomassie-stained 1D SDS-PAGE gels used in the second in-gel digestion. Fractions of acetone precipitated serum protein samples from a healthy sheep were used. One well in Gel A was loaded with BSA standard (arrow), and two other wells were loaded with 100 μg and 50 μg of protein each. Three wells in Gel B were loaded with 50 μg, 100 μg and 50 μg each of protein