Fig. 3.

Coomassie-stained 1D SDS-PAGE gels used for the third in-gel digestion. Fractions of pooled serum protein samples from six healthy sheep were used. In Gel A, one well was loaded with 200 μg of crude serum protein and the other well was loaded with 100 μg. Three 100 μg of serum protein for Gel B and C were loaded identically and treated as follows: one well was loaded with crude serum with a protease inhibitor (SP 100 μg + Pi), the second well had acetone precipitated serum and a protease inhibitor (SP 100 μg + Pi + Ac); the third well was loaded with crude serum only (SP 100 μg)