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. 2017 Jun 9;10:288. doi: 10.1186/s13071-017-2229-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of expression and antigenicity of rSjTOR-ed1. a Expression and purification of SjTOR-ed1 in E. coli. Cell extracts and fractions from E. coli BL21 (DE3) transformed with pET28-SjTOR-ed1 were separated by 12% SDS-PAGE. Lanes A and B: total extract of a clone before and after induction with one mM IPTG; Lanes C and D: supernatant and inclusion bodies, respectively, after lysis; Lane E: rSjTOR-ed1 protein purified from inclusion bodies through Ni-NTA His-binding resin. b Western blot analysis of the antigenicity of rSjTOR-ed1. Lane M: molecular mass markers; Lanes A, B and C: purified rSjTOR-ed1 was probed with anti-rSjTOR-ed1 mouse serum (positive control), serum from mice infected with S. japonicum (experiment group), and normal mouse serum (negative control), respectively