Table 1.

Bacteria and plasmids used in this study

Strains or plasmids	Description	Reference or source
Strains
E. coli LE392	Host strain of cosmid vector SuperCos I	Stratagene
E. coli DH5α	Host strain for general clone	Stratagene
E. coli ET12567/pUZ8002	Host strain for conjugation	[22]
E. coli BW25113	Host strain for PCR-targeting	[23]
S. syringae	Nocamycin-producing strain	NRRL
S. syringae MoS1001	ncmB deletion mutant strains originated from Saccharothrix syringae	This study
S. syringae MoS1002	ncmL deletion mutant strains originated from Saccharothrix syringae	This study
S. syringae MoS1003	ncmG deletion mutant strains originated from Saccharothrix syringae	This study
Plasmids
SuperCosI	Ampr, Kanr, cosmid vector	Stratagene
pIJ790	Cmlr, including λ-RED (gam, bet, exo) for PCR-targeting	[24]
pIJ773	Aprr, source of acc(3)IV and oriT fragment	[24]
pUZ8002	Kanr, including tra for conjugation	[25]
p5-C-9	Ampr, Kanr, harboring ncmLgene	This study
p2-H-12	Ampr, Kanr, harboring ncmG gene	This study