Fig. 4.

The expression of trehalose genes in the wild-type and Δpac-3 strains at normal growth pH (5.8) and alkaline pH (7.8). Cells from the wild-type and Δpac-3 strains were cultured at pH 5.8 for 24 h and shifted to pH 7.8 for 1 h. Mycelial samples were used to extract total RNA. Gene expression analysis was performed by RT-qPCR in the StepOnePlus™ Real-Time PCR system (Applied Biosystems) using the Power SYBR® Green and specific primers described in Additional file 1: Table S1. The β-tub gene was used as the reference gene, and the wild-type pH 5.8 was used as the reference sample. At least four biological replicates were performed in triplicate, and the data were analyzed using the relative quantification standard curve method. Bars indicate the standard deviation from the biological experiments. a, b, c, d: Letters above the bars indicate statistical significance; different letters indicate significant differences between two samples and similar letters indicate no significant difference between two samples at the same or different pH (Student’s t-test, P < 0.01)