Fig. 6.

The effects of Ca²⁺ on morphological growth of the Δpac-3 mutant strain. a The wild-type, Δpac-3 and Δpac-3 pac-3 ⁺ strains (10⁷ conidia) were inoculated onto Petri dishes containing solid VM medium plus 2% sucrose and CaCl₂ at final concentrations of 0, 10, 50, 100, 200 or 300 mM. Radial growth of the colonies was examined after 24 h growth at 30 °C. b Growth was measured and the percentage of growth was calculated taking the growth without Ca²⁺ as 100%. The results represent at least three independent experiments in duplicate. The asterisks indicate significant differences between the same strain cultured without Ca²⁺ and with different calcium concentrations, and the circles indicate significant differences compared to the wild-type strain at the same CaCl₂ concentration (Student’s t-test, P < 0.01). Error bars indicate standard deviation. c The effects of 0.05, 0.1, 1, 10 and 20 μM of calcineurin inhibitor (cyclosporin A, CsA) on the wild-type and Δpac-3 strains radial growth. d The effects of different concentration of CaCl₂ plus 0.05 μM CsA on the wild-type, Δpac-3 and Δpac-3 pac-3 ⁺ radial growth after 24 h at 30 °C