TABLE 2.
Identification and characterisation of spore-forming isolates
| Isolate no. | API50 CHBa
|
16S rRNA sequence analysis
|
Growthc
|
Plasmidsd | Hemolysise | |||
|---|---|---|---|---|---|---|---|---|
| Taxon | % ID/T | Closest known speciesb | % ID | 50°C | Anaerobiosis | |||
| 3 | B. megaterium 2 | 99.9/0.96 | B. megaterium | 99 | − | − | + | α |
| 10 | NI | B. macroides, B. simplex | 99 | − | − | − | γ | |
| 16 | B. megaterium 2 | 99.2/0.85 | B. megaterium | 99 | − | − | + | α |
| 17 | B. pumilus | 99.9/0.85 | B. pumilus | 100 | + | − | (−) | β |
| 19 | NI | Bacillus sp. strain VAN35 | 99 | − | − | (−) | γ | |
| 37 | B. subtilis | 95.3/1.00 | B. mojavensis, B. subtilis | 99 | + | +/− | − | “β” |
| 52 | Paenibacillus alvei, B. cereus 2 | 50.6/0.75, 39.4/0.56 | B. cereus group | 97 | + | + | + | β |
| 53 | NI | B. macroides, B. simplex | 99 | − | − | − | γ | |
| 56 | B. licheniformis | 98.0/0.87 | B. licheniformis | 99 | + | + | − | “α” |
| 62 | Brevibacillus brevis | 73.6/0.85 | B. silvestris | 95 | − | − | − | γ |
| 197 | B. pumilus | 99.2/0.82 | B. pumilus | 99 | + | − | − | β |
| 200 | B. subtilis | 95.3/1.00 | B. subtilis | 100 | + | +/− | − | “β” |
| 208 | NI | Bacillus sp. strain VAN35 | 99 | − | − | − | γ | |
| 210 | B. subtilis | 95.3/1.00 | B. subtilis | 100 | + | +/− | + | “β” |
| 235 | B. cereus 2 | 80.6/0.70 | B. cereus group | 99 | − | + | + | β |
| 241 | B. cereus 2 | 83.8/0.97 | B. cereus group | 99 | − | + | + | β |
| 243 | B. cereus 1 | 40.2/1.00 | B. cereus group | 99 | − | + | + | β |
| B. anthracis | 34.4/0.97 | |||||||
| B. mycoides | 25.2/0.99 | |||||||
| 257 | B. amyloliquefaciens | 49.0/0.74 | B. subtilis, B. atrophaeus, B. amyloliquefaciens, B. licheniformis | 99 | + | − | − | β |
| B. subtilis | 32.3/0.78 | |||||||
| B. licheniformis | 15.3/0.77 | |||||||
| 259 | B. licheniformis | 99.6/0.60 | B. clausii | 100 | −* | − | − | γ |
| 263 | B. pumilus | 99.9/0.96 | B. pumilus | 99 | + | − | − | β |
| 268 | NI | Bacillus sp. strain VAN35 | 99 | − | − | + | γ | |
| 278 | B. subtilis | 95.3/1.00 | B. subtilis, B. mojavensis, B. atrophaeus, B. licheniformis | 99 | + | + | − | β |
| 285 | B. subtilis | 97.1/0.74 | B. subtilis, B. mojavensis, B. atrophaeus, B. licheniformis | 99 | + | + | + | “α” |
| 287 | NI | Bacillus sp. strain N6 | 99 | − | − | + | γ | |
| 306 | B. licheniformis | 90.5/0.37 | B. pumilus | 99 | + | − | − | β |
| 317 | B. firmus | 86.6/0.54 | B. firmus | 98 | − | − | − | γ |
| Brevibacillus laterosporum | 12.6/0.45 | |||||||
| 340 | B. licheniformis | 99.9/0.74 | B. sonorensis, B. licheniformis | 99 | + | + | + | “α” |
| 344 | B. pumilus | 99.9/0.83 | B. pumilus | 99 | + | − | − | β |
| 345 | B. licheniformis | 99.9/0.98 | B. licheniformis | 99 | + | + | + | V |
| 369 | NI | Bacillus sp. strain VAN35 | 99 | − | − | + | γ | |
| 370 | NI | B. clausii | 99 | −* | − | − | γ | |
NI, the APILAB Plus software was unable to find an acceptable identity (ID).
Closest known species relative with BLAST (0 to 100%); underlined, closest known species relative with the Ribosomal Database Project II; bold, isolates for which the full sequence of the 16S rRNA was determined; for all other isolates, analysis is based on partial sequences of 600 to 800 nt. B. cereus group is listed when similar sequence identities were found for two or more species of the B. cereus group.
*, positive after 48 h of incubation. +/−, limited growth in the form of tiny colonies.
Parentheses indicate that the presence of a large plasmid is not clear.
Weak signals are in quotation marks. With the exception of hemolysis (performed twice), testing for growth at 50°C, anaerobiosis, and the presence of plasmids were repeated at least three times. V, variable result.