Table 1.
Glycan Sites and Heterogeneity of Glycans Attached to Glycopeptides Obtained from Clusterin Present in OC Ascites Fluids
| Sample #2 - desialyiated | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| PID | Site | Sequence | Glycans | Score | structure | composition | # peptides | total# glycopeptides in site | % heterogeneity |
| 2 | 684133 | N86 | K.KKEDALN[+2133.77171]ETR.E | HexNac(5)Hex(6)Fuc(1)NeuAc(0) | 161.8 |
|
H6N5F1S0 | 3 | 27% | |
| 5 | 684333 | N86 | K.KKEDALN[+2133.77171]ETR.E | HexNac(5)Hex(6)Fuc(0)NeuAc(0) | 213.9 |
|
H6N5F0S0 | 3 | 27% | |
| 12 | 650092 | N86 | K.KKEDALN[+1622.58161]ETR.E | HexNac(4)Hex(5)Fuc(0)NeuAc(0) | 235.7 |
|
H5N4F0S0 | 5 | 11 | 45% |
| 24 | 709068 | N291 | R.TVC[+57.02146]REIRHN[+1606.58669]STGC[+57.02146]LR.M | HexNac(4)Hex(4)Fuc(1)NeuAc(0) | 129.2 |
|
H4N4F1S0 | 2 | 13% | |
| 26 | 683653 | N291 | R.TVC[+57.02146]REIRHN[+1460.52878]STGC[+57.02146]LR. M | HexNac(4)Hex(4)Fuc(0)NeuAc(0) | 128.8 |
|
H4N4F0S0 | 2 | 13% | |
| 1 | 683013 | N291 | R.HN[+2352.84600]STGC[+57.02 146]LR.M | HexNac(6)Hex(7)Fuc(0)NeuAc(0) | 83.1 |
|
H7N6F0S0 | 1 | 6% | |
| 8 | 649370 | N291 | R.HN[+1987.71380]STGC[+57.02 146]LR.M | HexNac(5)Hex(6)Fuc(0)NeuAc(0) | 238.4 |
|
H6N5F0S0 | 3 | 19% | |
| 11 | 603402 | N291 | R.HN[+1768.63952]STGC[+57.02 146]LR.M | HexNac(4)Hex(5)Fuc(1)NeuAc(0) | 227.7 |
|
H5N4F1S-0 | 1 | 6% | |
| 18 | 603002 | N291 | R.HN[+1622.58161]STGC[+57.02 146]LR.M | HexNac(4)Hex(5)Fuc(0)NeuAc(0) | 463.2 |
|
H5N4F0S0 | 7 | 16 | 44% |
| Sample #4 - same as #2 except not desialylated | |||||||||
| 1 | 715191 | N86 | K.KKEDALN[+3007.05796]ETR.E | HexNac(5)Hex(6)Fuc(1)NeuAc(3) | 30 |
|
H6N5F1S3 | 2 | 40% | |
| 3 | 715231 | N86 | K.KKEDALN[+2861.00005]ETR.E | HexNac(5)Hex(6)Fuc(0)NeuAc(3) | 30 |
|
H6N5F0S0 | 1 | 20% | |
| 5 | 641812 | N86 | K.KKEDALN[+2204.77244]ETR.E | HexNac(4)Hex(5)Fuc(0)NeuAc(2) | 124.2 |
|
H5N4F0S2 | 2 | 5 | 40% |
| 4 | 607891 | N291 | R.HN[+2278.80922]STGC[+57.02 146]LR.M | HexNac(5)Hex(6)Fuc(0)NeuAc(1) | 104.1 |
|
H6N5F0S1 | 1 | 17% | |
| 7 | 608131 | N291 | R.HN[+1913.67702]STGC[+57.02 146]LR.M | HexNac(4)Hex(5)Fuc(0)NeuAc(1) | 347.6 |
|
H5N4F0S1 | 5 | 6 | 83% |
| Sample #6 - HSA/IgG depleted sample | |||||||||
| 1 | 413677 | N291 | R.EIRHN[+2204.77244]STGC[+57.02146]LR.M | HexNac(4)Hex(5)Fuc(0)NeuAc(2) | 132.9 |
|
H5N4F0S2 | 2 | 100% | |
| 3 | 363539 | N291 | R.HN[+2204.77244]ST GC[+57.02146]LR.M | HexNac(4)Hex(5)Fuc(0)NeuAc(2) | 349.2 |
|
H5N4F0S2 | 3 | 5 | 100% |